Monday, June 29, 2015

NUTRITION STUDENTS' ACTIVITIES

NUTRITION

STUDENTS' ACTIVITIES

Carbohydrates are the products of photosynthesis. Simple sugars are the first to be formed but are immediately converted into starch, the first visible product of photosynthesis. It is therefore possible to find out if a plant has carried out photosynthesis by testing for starch. Presence of starch implies that photosynthesis has occurred.
Several practical activities should be carried out in order to learn more about the nature and factors necessary for photosynthesis. These activities include the following:
Experiment 1. Testing for Starch
Experiment 2. Showing that carbon dioxide is necessary for photosynthesis.
Experiment 3. Showing that light is necessary for photosynthesis.
Experiment 4. Showing that chlorophyll is necessary for photosynthesis.
Experiment 5. Showing that oxygen is given off during photosynthesis.

EXPERIMENT 1: TESTING FOR STARCH


Aim: - To test a leaf for starch.


Apparatus and Materials

Beaker, boiling tube, heat source, test tube holder
Iodine solution, ethanol, water and a leaf that has been exposed to light.

Procedure

Half fill a beaker with water and boil the water. Place the leaf in boiling water for one or two minutes. This denatures the enzymes and stops chemical reactions. Put the leaf into a boiling tube and pour enough ethanol to cover the leaf. Place the boiling tube into the beaker of boiling water. A water bath is used to prevent accidents that may arise from alcohol being inflammable. Remove the flame when the ethanol boils.
Re-introduce the flame when the ethanol stops boiling. Repeat until the leaf is decolourised.

Diagram to illustrate boiling a leaf in alcohol using a water bath.
(Testing a leaf for starch)
- When the chlorophyll has been removed (the leaf looks white) remove the leaf from ethanol (the leaf will be brittle).
- Dip the leaf in water to soften it and make it permeable.
- Using a pipette, add dilute iodine solution to the leaf surface.
Student’s discussion:
1. When boiling the leaf in ethanol, what happened to the colour of ethanol? Explain your observation.
2.What colour did the leaf turn when you added iodine?
3. What do these results show?

 

Adaptations of a leaf for photosynthesis

Adaptations of a leaf for photosynthesis


Transverse section of a leaf showing some of the adaptations

Cuticle: - Is non-cellular, water proof and transparent. It allows light to pass through.
Upper epidermis: - Is a single layer of cells on the upper surface of a leaf. It allows light to pass to the cells below.
Palisade mesophyll: - has cells vertically arranged such that many can fit into a small space. The cells have large number of chloroplasts.
Chloroplasts:- contain chlorophyll for absorbing sunlight.
Spongy mesophyll: - Have large air spaces for fast diffusion of gases to and from the photosynthesizing cells. The cells have few chloroplasts.
Veins (Vascular bundles): - They act as drain pipes distributing raw materials to the leaves and conducting away manufactured food to other parts of a plant.
Stoma: - A pore that allows gaseous exchange to take place. Carbon dioxide enters and oxygen leaves the leaf through stomata.
Thin lamina: - Provides a short distance over which diffusion of gases take place.
Broad lamina: - Some leaves have a broad lamina which provides a large surface area for absorbing sunlight energy

MECHANISM OF PHOTOSYNTHESIS

MECHANISM OF PHOTOSYNTHESIS

The process of photosynthesis is summed up in the word and chemical equation below:
carbon dioxide + Water  Glucose + Oxygen
(Raw materials)         sun light and   (Products)
                               Chlorophyll.

                              Sun light
6CO2 (g)+ 6H20 (l) C6H12O6 + 6O2
                            Chlorophyll

Photosynthesis occurs in two phases; namely light- dependent stage (light reaction/ stage) and light independent stage (dark reaction/stage.)
Light – dependent stage
Radiant energy is absorbed by chlorophyll. The chlorophyll is activated and it converts light energy into chemical energy in the form of ATP. [Adenosine Triphosphate]
Water is split into hydrogen ions and hydroxyl ions.

      Light
H2O   [ H ]+ + [OH]¯
     Chlorophyll

Oxygen gas is formed from reactions, involving hydroxyl ions.
The products of the light dependent stage are ATP, H+ ions and oxygen.

Light independent stage
The hydrogen ions and chemical energy that were produced during the light dependent phase are used to reduce carbon dioxide to form glucose.

MACRO AND MICRO NUTRIENTS

Table : Macro and micronutrients and their uses.
Macro nutrients
Uses
Deficiency
Nitrogen
Amino acid and protein synthesis
- Chlorosis with purple colourings
Phosphorus

Protein formation
- Reddish purple leaves and stunted growth.
Sulphur
Formation of certain amino acids
- Stunted growth
- yellow patches on leaves
Calcium
Formation of cell wall (middle lamella)
- Poor development of leaves at shoot apex
Potassium
Concerned with synthesis of carbohydrates and protein metabolism in young leaves.
- Chlorosis, and yellowing of leaves at the margins, tips.
- Retarded growth.
Magnesium
Chlorophyll formation. Facilitates enzyme activity.
- Chlorosis of older leaves. - Stunted growth.
Iron
Required for the formation of chlorophyll but not part of the molecule.
- Chlorosis with pale leaves

Micro nutrients

Cobalt
Copper
Zinc
Manganese
Boron
They are necessary for the manufacture of enzymes.
- Stunted growth of leaves.

NUTRITION Introduction

NUTRITION
Introduction
Organisms obtain food in various ways so as to survive and carry out physiological processes like respiration, growth, osmo-regulation among others. Organisms having chlorophyll like plants and protests like Euglena make food by combining inorganic substances namely water and carbon dioxide to make carbohydrates in a process called photosynthesis. They use energy from the sun. Some organisms use energy from the oxidation of certain substances to make food in a process called chemosynthesis. Organisms that make their own food are referred to as autotrophs.
Some organisms obtain complex food substances made by other organisms and break them down to simple substances while others make their own food from simple inorganic substances. Some organisms obtain food by breaking down dead organic substances. These are called saprophytes and the type of feeding is called saprophytism e.g fungi feeding on a dead decaying wood.
Others obtain food by associating with other organisms in particular kinds of relationships. Some organisms obtain food by associating with others not of their own kind in a relationship called parasitism.
In this relationship, the organism that benefits is called a parasite while the other does not benefit and is harmed; it is called a host e.g a tick on a cow. In other relationships, both organisms benefit from the relationship. This is called mutualism e.g the gut bacteria. In some relationships, one may benefit or not. This is called commensalism and the organisms involved are called commensals.
All relationships where two organisms live together are called symbiosis.
Organisms which obtain food by breaking down complex food substances to simple absorbable molecules are called heterotrophs.

NUTRITION IN GREEN PLANTS
Green plants differ from other organisms because they take in simple materials such as carbon dioxide, water and mineral salts (i.e. nitrates, phosphates and sulphates) to build more complex substances including carbohydrates, oils and proteins. The building up of carbohydrates takes place in the presence of the sunlight in a process called photosynthesis. Therefore, the raw materials for photosynthesis are water and carbon dioxide. During photosynthesis plants give off oxygen according to the equation below.
                  Light energy
6H20 +6C02       C6 H1206 + 602
                  Chlorophyll

Some carbohydrates are later changed into lipids and proteins.
This unit therefore deals with: -
• The process of photosynthesis.

• Rate of photosynthesis.

• Factors affecting photosynthesis.

• The structure of a leaf and its adaptations to photosynthesis.

• Experiments on photosynthesis.

• The importance of major plant nutrient elements.

Factors affecting the rate of photosynthesis

When plenty of carbon dioxide, sunlight and water are provided to a plant, photosynthesis will be at its maximum rate; sometimes the rate of photosynthesis is not as high as expected due to inadequacy of factors that include light intensity, water, temperature and carbon dioxide concentration.
Providing plenty of each of these factors to a plant increases the rate of photosynthesis. However, if one of these factors is not adequate, the rate of photosynthesis may become low. A factor that is inadequate is called a limiting factor.
Light intensity: in case of dim light, the rate of photosynthesis is low. As light intensity increases, the rate of photosynthesis increases.
Carbon dioxide concentration: the more carbon dioxide a plant is given, the faster is the rate of photosynthesis, until a maximum is reached.
Temperature: photosynthesis is an enzyme-controlled process. Increase in temperature increases the rate of photosynthesis while a decrease lowers it.
Size of stomatal aperture: This regulates the amount of carbon dioxide entering a plant which it uses for photosynthesis. If the stomata are closed then photosynthesis cannot take place and when they are open, carbon dioxide enters and the rate of photosynthesis increases.

Mineral salts are necessary for normal plant growth

Several elements are required for normal plant growth and development.
Some of these elements are Carbon, Hydrogen and Oxygen.
Other elements are Nitrogen, Sulphur, Phosphorus, Potassium, Calcium and Magnesium.
The last category of important elements includes Iron III, Manganese, Zinc, Boron and Molybdenum. These elements in the last category are called trace elements because they are needed in small amounts for normal growth. Apart from the first category, the plant obtains the nutrients it needs from the soil.
The effects of these chemical elements can be discovered by growing plants in water solutions containing balanced amounts of salts necessary for healthy plant growth. This solution is called a culture solution. If any of the elements is left out; its effect can be observed.
Table II: Shows the importance of certain elements are necessary for normal plant growth.
CONTENTCULTURE EXPERIMENT RESULTSFUNCTION OF ELEMENT
Complete solution Normal healthy growth
-
Distilled water.

Virtually no growth
-
No Nitrogen Very little growth
Component of all proteins
No phosphorusThin lanky growth, poor root development and reddish leavesComponent of certain enzymes and in the nucleus is substances like DNA
No Sulphur Some growth but less than it would be in complete solution.
Present in certain proteins
No PotassiumLittle growth. Leaves turn orange brown.Needed in cell formation
No CalciumStunted growthNeeded in cell wall formation
No MagnesiumStunted growth and leaves turn yellow.Chlorophyll contains magnesium.
No Iron
Poor growth,leaves turn yellow.Needed in Chlorophyll synthesis

Transport of materials in Plants



TEACHER’S GUIDE

BIOLOGY                 2. Form: S3

UNIT 2: TRANSPORT OF MATERIALS IN PLANTS

Introduction

If plants and animals are to survive then they must have evolved ways to transport the nutrients to all of their tissues.

The nutrients they use to live and to grow come from their environment.

In plants the main nutrients are carbon dioxide from the air and minerals from the soil. They use these to make the substances they need to maintain themselves. In green plants the carbon dioxide is taken from the air through their leaves. The minerals they need are taken up through their roots, as is water which is also essential for life.

Animals take in food through their mouths or, in very small organisms, they may be absorbed through their skin.

The challenge for plants is to evolve ways to transport their nutrients from the surface of the plant to cells in the middle of the plant that also need to be nourished. Animals also must have systems for moving the food they take in to their stomachs or through their skin (for small organisms) to the rest of their bodies. They must also have ways to transport waste products out of their bodies.

Very small organisms use a method called diffusion to move materials. Nutrients that are dissolved or suspended in water are absorbed through their skins. Once they have been taken in to the plant or animal, they gradually percolate or diffuse through their tissues towards the cells in the middle. The problem is that the rate of diffusion of substances through the tissues may be slow, so it only works well with small organisms like protozoa, single-celled plants and jellyfish. Bigger organisms cannot be kept alive by diffusion alone, because the cells that are further away from the source of nutrients would die. So size is a limiting factor, if the organism depends only on diffusion as its means of transporting nutrients.

Larger organisms need more complex systems to transport nutrients around their bodies. In the case of mammals, for example, they have a heart and blood vessels that allow fluids containing nutrients to be transported quickly and efficiently.

BRIEF DESCRIPTION OF UNIT OF TEACHER SUPPORT MATERIAL:

This unit deals with;
  • Structure of root hairs and stem.
  • Movement of water, minerals, salts and manufactured food through a transport system.
  • Structure and functions of the root hairs, stem and roots in relation to transport
  • Diffusion, osmosis, and plant cell relation: plasmolysis. Flaccidity and turgidity.
  • Active transport
  • Opening and closing of stomata
  • Transpiration and factors affecting transpiration.
  • Translocation
  • Storage organs and food nutrients.
           
SUMMARISE THE MAIN CONTENT AND CONCEPTS THAT THE TEACHER SHOULD EMPHASISE IN TEACHING THE SUB-TOPIC:

  • Transverse section of root, and stem.
  • Simple structure of xylem and phloem and relationship between structure and function.
  • Movement of water and solutes from soil to leaves.
  • Diffusion, osmosis: Experiments on diffusion and osmosis.
  • Transpiration and factors affecting it.

LIST ITEMS OF TEACHING/LEARNING MATERIALS:
(Worksheet, stimulus activity, experiments, items of evidence, statistics, texts, pictures, diagrams, graphs)
Chart with transverse section of a stem and root, xylem and phloem.
  • Knife/razor blade/scapel.
  • Microscope, microscopes slide and cover slips.
  • Simple potometer, polyethene bag.
  • Stain e.g. iodine.
  • Anhydrous copper II sulphate/blue cobalt chloride paper.
  • Locally available storage organs (cassava, potatoes, sugar cane etc.)
  • Text books stain e.g. iodine
  • Common salt
  • Prepared slides of the stem, root, and stomata.
  • Animation of diffusion molecules from an area of high concentration to low concentration.

Worksheet for practical /experimental activity.
Aim:
To show that a plant transpires.
Materials and apparatus.
-        Potted plant or attached shoot
-        Polythene bag
-        Thread/rubber bands
-        Cobalt chloride paper,/anhydrous copper II sulphate

Procedure

Cover a branch or shoot with a polythene bag. Tie around the stem with a string or rubber band firmly. (See diagram below)
Place the set up under sunlight and leave for 2 hours.
Remove the polyethene bag and collect the liquid that may have gathered in the bag water.
Test the liquid with anhydrous copper II sulphate/cobalt chloride paper
Observation:
 A colourless liquid collects in the bag.
 The white anhydrous powder copper II sulphate powder turns blue/blue cobalt chloride paper pink/ in the control experiment there is no water.
Conclusion;
A plant gives off water in form of vapour

Learners’ activity.

1.                 a)   Why isn’t the pot enclosed?
b)     Why is the   polythene bag tied firmly?




Answers to learner’s activity.

       a)     To ensure that any moisture   that is collected is lost from aerial parts but not from the soil.
b)     To prevent moisture from the atmosphere getting into the polythene bag.

Evaluation questions.

  1. Which of the following tissues conducts water and also provides mechanical support to a plant
  1. Phloem
  2. Xylem
  3. Cambium
  4. Cortex
 
  1. The figure below shows a transverse section of a dicot stem. Study it and answer the questions that follow.
a)     Name the parts labeled P to U.
b)     State the functions of each of the parts labeled S and T.
  1. State two differences between diffusion and osmosis

Answers to evaluation questions
1. B
2. a)
         P – Cortex
        Q - Pith
        R  - Cambium
        S – Xylem
        T - Phloem
        U – Epidermis
 
   b) S – Conducts water and mineral salts up the plant
        T – Conducts manufactured food from leaves to other parts of a plant.

3.

DIFFUSION
OSMOSIS
Solutes move from high concentration to low concentration
Water moves from high to low concentration
Membranes not involved
Semi-permeable membrane involved




Worksheet for practical activity /Experiment II

Aim:
To demonstrate osmosis in living tissues.

Materials/ and apparatus.

Concentrated solution of salt/sugar, potato/unripe paw paw.
Water, basins/ trough/sauce pan, knife
Procedure:
Peel two potatoes to expose the living tissue.
Scoop them to form a cup as shown below.
Place concentrated solution of salt/sugar in cups A and water in cup B.
Mark the levels in the potato cups.
Place the potato cups into Petri dishes of water.
Mark the levels of water in the Petri dishes.
Observe the levels in the Petri dishes and the cups after 6- 24 hours.
Observations
Level of the solution rises in   cup A while the level of water in the Petri dish falls.
Level of water in cup B   and the Petri dish does not change.

Conclusion;
Osmosis takes place in living tissues.

TEACHER’S GUIDE   Include essential teacher information on separate pages: topic notes, learning objectives, organisational advice and tips, answers to student exercises, advice on assessment/evaluation, marking and exam preparation, suggested follow-up and extension work, useful textbook references and other resources).


References:
  • Beckett B.S (1982) Biology: A modern introduction (2nd Edition) London, Oxford University Press.
  • Hayward. G. Semakadde I and Ochiro. E. (2002) Macmillan secondary Biology, London, Macmillan.
  • Mackean .D.G (1973) Introduction to Biology. London, Evans Brothers, UK.
  • Stone R. H and cozens A, B (2002). New Tropical Biology 3rd edition. London (UK) Longman

Useful tips:

The teacher should ensure that control experiments are set up in order to obtain results.
Involve students in suggesting control experiments.

SAMPLE EXAMINATION QUESTIONS


SECTION A: OBJECTIVES


  1. Which of the following processes for movement of molecules in and out of cells specifically refers to water molecules?
    1. Diffusion
    2. Active transport.
    3. Osmosis
    4.  Phagocytes
  2. Which of the following processes requires energy?
    1. Translocation.
    2. Diffusion
    3. Osmosis
    4. Active transport.

  1. In which underground organ does cassava store starch?
    1. Stem tuber.
    2. Root tuber
    3. Rhizome
    4. Corn

SECTION B: STRUCTURED QUESTION

.
1.     The table below shows the distribution of stomata on the leaves of plants A and B, which live in different habitats. Study the table and answer the questions that follow:
           
Leaf
No. of stomata on upper surface
No. of stomata on lower surface
A

150
02
B

35
100

a)     Name the habitat for each leaf.
              Leaf A………………………………..
              Leaf B……………………………….                                                 (2 marks)
b)       
                           i.          State the difference in the distribution of stomata in plants A and B.
…………………………………………………………………………………
…………………………………………………………………………………
………………………………………………………………………………..
…………………………………………………………………………………
                                                                                                     (11/2  marks)
                         ii.          Give reasons for the difference stated in b (i) above.
………………………………………………………………………………
………………………………………………………………………………..
……………………………………………………………………………….
………………………………………………………………………………..
……………………………………………………………………………….
……………………………………………………………………………….
……………………………………………………………………………….
                                                                                                         ( 3 marks)
c)     Give the advantages of transpiration to a plant.
……………………………………………………………………………………….
………………………………………………………………………………………
……………………………………………………………………………………..
                                                                                                                     (2 marks )
d)     State any two adaptations of plants that enable them to survive in desert areas.
………………………………………………………………………………….
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
                                                                                                                (2 marks)

SECTION C: ESSAY/LONG ANSWER QUESTION.
1. a) How does water move from the soil to xylem of a root?            (7 marks)
    b) Give the adaptations of root hairs to absorption of water.          (2 marks)
    c)  Name four environmental factors that affect the rate of transpiration

ANSWERS TO SAMPLE QUESTIONS.

SECTION A
    1. C
    2. D
    3. B

SECTION B

1. a)   Leaf A - Water
          Leaf B – Well-watered soil/land.
    b)
i.                 The leaves of plant A have more stomata on the upper than the lower surface whereas the leaves of B have more stomata on the lower than the upper surface.
ii.               Plant A has more water available to it than plant B. A needs to lose excess water. B needs to conserve water. In A the stomata are exposed to direct sunlight, promoting rapid evaporation. In B the stomata are sheltered from direct sunlight, thus less evaporation.
c) - Cools the plant.
    -Enables absorption of water and mineral salts.
    -Enables upward movement of water and mineral salts     (any two points)                          
   d) -    Thick cuticle on leaves to reduce water loss
-        Leaves modified to spines to reduce water loss.
-        Leaves have small surface area to reduce water loss.
-        Extensive superficial root system to absorb water over a large area.
-        Deep roots absorb water from deeper layers of soil.
-        Succulent stems or leaves for storage of water.
-        Stomata opening at night and closing during the day to reduce water loss.
-        Stomata sunken in pits to reduce water loss.          (any two points)

 

SECTION C

1.
(a)    Root hairs absorb water from the soil by osmosis because the sap in the root hair is more concentrated than the soil solution. The sap of the root hair cell becomes less concentrated than that of the neighboring cortical cell, which in turn draws in water from it by osmosis. Water moves in the same way across the cortex up the xylem vessel. It then diffuses into the xylem vessel.
(b)   Root hairs are thin and long to increase the surface area for absorption
Root hairs are numerous, further increasing the surface area of absorption
(c)    Temperature: High temperatures favour high rates of transpiration while low temperatures favour low rates.
Relative humidity: the rate of transpiration is high at low relative humidity and low at high relative humidity.
Light intensity: At high light intensity the rate of transpiration is high. At low light intensity the rate is low
Wind: The rate of transpiration is higher in windy conditions than in still air.
Atmospheric pressure: The rate of transpiration is higher at low atmospheric pressure.

Glossary:

Xylem: Tissue that conducts water and mineral salts from the roots to the leaves.
Phloem: Tissue that conduct food materials from the leaves to the other parts of a plant

Semi-permeable membrane: /selectively

Is one that allows only solvent molecules to pass through while preventing the passage of solute molecules.
Flaccid: Describes a cell that has lost its turgidity and is relatively soft due to loss of water.
Plasmolysis: The loss of water from a plant cell to the extent that the protoplasm pulls away from the cell wall. Continued plasmolysis leads to wilting.
Transpiration: Is loss of water vapour from aerial parts of a plant to the atmosphere.
 Occurs mainly through the leaves.
Cohesive force: Is force of attraction between like molecules or molecules of the same substance.
Adhesive force: Is force of attraction between unlike molecules or molecules of different substances.
 Translocation:  Is movement of minerals and chemicals compounds within a plant 
 It involves movement of water and mineral salts through the xylem and manufactured food through the phloem.
Turgor:    Is a condition in a plant cell when its vacuole is distended with water pushing the protoplasm against the cell wall.